Appendix A to Part 449 - Sampling Protocol for Soluble COD
40:32.0.1.1.24.4.5.1.20 : Appendix A
Appendix A to Part 449 - Sampling Protocol for Soluble COD
This sampling protocol applies only to samples collected for use
in measurement of COD when demonstrating compliance with the
regulations set forth in this part. Collect a representative sample
of the effluent from the airport deicing treatment system, based on
the discharge permit requirements (e.g., a grab sample or a
composite sample). Because only the COD sample is filtered, do not
use in-line filters if collecting a sample with a compositing
device.
A. Grab Samples
1. Cap the container and shake the grab sample vigorously to mix
it. Remove the plunger from a 10-milliliter (mL) or larger
Luer-lock plastic syringe equipped with an Acrodisc Luer-lock
filter containing a 1.5-μm glass fiber filter (Whatman 934-AH, or
equivalent), and fill the syringe body with sample.
2. Replace the plunger and filter the sample into a clean 50-mL
screw-cap glass, plastic, or fluoropolymer bottle.
Note:
If testing is being done in the field, or with a test kit
product (e.g., Hach Method 8000), the filtrate may be collected in
the test kit vial or container.
3. Additional 10-mL volumes of sample may be filtered and the
filtrate added to the same sample bottle. This additional volume
may be used to repeat sample analyses or to prepare Quality Control
(QC) samples, as needed.
4. Unless the filtered sample will be analyzed within 15
minutes, preserve the filtered sample with H2SO4 to pH <2. Cap
the bottle and label with the sample number. Place in a cooler on
ice prior to shipping.
5. Once at the analytical laboratory, the sample must be stored
at ≤6 degrees Celsius and analyzed within 28 days of collection
(see the requirements for COD in Table II at 40 CFR part 136).
6. Analyze the sample using a method approved for COD in Table
IB at 40 CFR part 136.
Note:
Because this procedure is specific to this point source
category, it does not appear by name in 40 CFR part 136.
7. Report the sample results as Soluble COD in units of
milligrams per liter (mg/L). There is no Chemical Abstracts Service
(CAS) Registry Number for soluble COD.
B. Composite Samples
1. If the sample will be analyzed in a fixed laboratory (as
opposed to field testing), transfer at least 50 mL of well-mixed
sample from the compositing device into a clean 50-mL screw-cap
glass, plastic, or fluoropolymer bottle. Preserve the sample with
H2SO4 to pH <2. Cap the bottle and label with the sample number.
Place in a cooler on ice prior to shipping.
2. Once at the analytical laboratory, the sample must be stored
at ≤6 degrees Celsius and analyzed within 28 days of collection
(see the requirements for COD in Table II at 40 CFR part 136).
3. Prior to analysis, remove the sample from cold storage and
allow it to warm to room temperature. Shake the sample vigorously
to mix it.
4. Remove the plunger from a 10-mL or larger Luer-lock plastic
syringe equipped with an Acrodisc Luer-lock filter containing a
1.5-μm glass fiber filter (Whatman 934-AH, or equivalent), and fill
the syringe body with sample.
5. Replace the plunger and filter the sample into a clean COD
vial or other suitable container.
6. Additional 10-mL volumes of sample may be filtered and the
filtrate added to separate containers, as needed, to provide
samples for repeat analyses or to prepare QC samples.
7. Analyze the sample using a method approved for COD in Table
1B at 40 CFR part 136.
Note:
Because this procedure is specific to this point source
category, it does not appear by name in 40 CFR part 136.
8. Report the sample results as Soluble COD in units of mg/L.
There is no CAS Registry Number for soluble COD.